Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity

نویسندگان

  • Farzad Kobarfard Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Hasan Hooshdar Tehrani Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Hossein Vatanpour Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Hossein Zolfagharian Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Mohammad Hossein Heydari Proteomics Research Center, Paramedical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Toktam Mehdizadeh Kashani Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده مقاله:

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 µM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.

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منابع مشابه

Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibi...

متن کامل

partial fractionation of venoms from two iranian vipers, echis carinatus and cerastes persicus fieldi and evaluation of their antiplatelet activity

platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of cerastes persicus fieldi and echis carinatus were investigated. the partial fractionation was performed on a sephadex g-100 column. two fractions separated from cerastes persicus fieldi showed anti platelet aggregation activity on adp (200 µm)-induced platelet aggregation (ca 80% inhibi...

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Isolation and partial purification of anticoagulant fractions from the venom of the Iranian snake Echis carinatus.

Many snake venoms comprise different factors, which can either promote or inhibit the blood coagulation pathway. Coagulation disorders and hemorrhage belong to the most prominent features of bites of the many vipers. A number of these factors interact with components of the human blood coagulation. This study is focused on the effect of Echis carinatus snake venom on blood coagulation pathway. ...

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عنوان ژورنال

دوره 11  شماره 4

صفحات  1183- 1189

تاریخ انتشار 2012-07-15

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